A4 Conference proceedings

Quantitative multiplex CARS micro/spectroscopy in congested spectral regions


LUT Authors / Editors

Publication Details
Authors: Müller Michiel, Rinia Hilde A, Vartiainen Erik M, Bonn Mischa
Publication year: 2007
Language: English
JUFO-Level of this publication:
Open Access: Not an Open Access publication

Abstract
A novel procedure is developed to describe experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach - exemplified here with multiplex CARS data of the phase transition of DMPC LUVs (Fig. 1) - makes use the maximum entropy method for phase retrieval. The retrieved imaginary part of the nonlinear susceptibility ( { (3)} Im χ ) is shown to be equal to the spontaneous Raman spectrum. The phase retrieval procedure does not influence the noise contained in the spectra. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel procedures enables extraction of vibrational information from multiplex CARS data without the use of a priori information of the sample. Various applications are discussed, including high resolution multiplex CARS microscopy on cells. Figure

Last updated on 2017-22-03 at 16:00