A1 Journal article (refereed), original research

Profiling of water-soluble carbohydrates in pine and spruce extracts by capillary zone electrophoresis with direct UV detection


Publication Details
Authors: Kaijanen Laura, Metsämuuronen Sari, Reinikainen Satu-Pia, Pietarinen Suvi,
Jernström Eeva

Publication year: 2015
Language: English
Related Journal or Series Information: Wood Science and Technology
JUFO-Level of this publication: 1
Open Access: Not an Open Access publication

Abstract
Monitoring wood-related biorefinery process streams is important as thecomposition of raw material and its variations have a great impact on the productionoutcome as well as on the process control. In this paper, the applicability of a simpletype of capillary zone electrophoresis method to profiling heterogeneous woodbasedsamples was studied by grouping the peaks in the overall electropherograms.A comparison between 210 and 270 nm as an example confirmed the identificationof a monosaccharide peak in the carbohydrate electropherogram. The analyticalmethod applied in this study was found suitable, firstly, in profiling the samplesbased on correlation of multiple detection wavelengths and, secondly, in characterizingthe total carbohydrate composition by grouping detected peaks by thecompound type. Compounds were detected in their extracted form without timeconsumingpretreatment procedures. Differences in the raw material and processconditions were successfully identified by comparing characteristic peaks in thesample electropherograms of an example case.

Last updated on 2017-22-03 at 14:32