A1 Journal article (refereed), original research

Determination of xylo-oligosaccharides in enzymatically hydrolysed pulp with liquid chromatography and capillary electrophoresis


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Publication Details
Authors: Metsämuuronen Sari, Lyytikäinen Katja, Backfolk Kaj, Sirén Heli
Publication year: 2013
Language: English
Related Journal or Series Information: Cellulose
Volume number: 20
Issue number: 3
ISSN: 0969-0239
JUFO-Level of this publication: 2
Open Access: Not an Open Access publication

Abstract
Three different com. β-1,4-endoxylanase prepns. were used to hydrolyze bleached kraft pulp. Xylo-oligosaccharides in the produced filtrates were sepd. and quantified using both high performance liq. chromatog. (HPLC) and capillary electrophoresis (CE). All the detns. were performed without sample derivatization. The anal. methods were used to highlight the differences between the enzymes behavior in terms of hydrolyzates, but also to est. the productivity of xylo-oligosaccharides from kraft pulp when the bleached material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the pulp material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concns. obtained after acid hydrolysis. The CE data showed the same trend, but much lower concns. were identified than with HPLC. At the same time the HPLC method was able to sep. only small oligosaccharides, whereas CE could be used for sepn. of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft pulp.

Last updated on 2017-22-03 at 15:05