A1 Journal article (refereed), original research

Lysine-based dendrimer with double arginine residues


Open Access publication

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Publication Details
Authors: Sheveleva N., Markelov D., Vovk M., Mikhailova M., Tarasenko I., Tolstoy P., Neelov I., Lähderanta E.
Publisher: Royal Society of Chemistry: Open Access
Publication year: 2019
Language: English
Related Journal or Series Information: RSC Advances
Journal name in source: RSC Advances
Volume number: 9
Issue number: 31
Start page: 18018
End page: 18026
Number of pages: 9
ISSN: 2046-2069
JUFO-Level of this publication: 1
Open Access: Open Access publication

Abstract

Due to their well-defined structure, multivalency, biocompatibility, and low toxicity, lysine dendrimers can be used as safe and efficient nanocarriers for drug and gene delivery. One useful strategy for improving the gene delivery properties of dendrimers is modification with arginine amino acid (Arg) residues. Incorporation of Arg residues could be favorable for the enhancement in transfection efficiency of lysine based dendrimers. In this work, we have synthesized a new second-generation poly-L-lysine dendrimer with repeating units containing two linear Arg residues between neighboring lysine branching points (Lys-2Arg dendrimer) and studied its physicochemical properties. We confirmed the structure of Lys-2Arg dendrimer using various one- and two-dimensional 1H and 13C NMR spectroscopy methods. Comparison of T1H relaxation data for Lys-2Arg and Lys-2Lys dendrimers showed that the replacement of double Lys residues with double Arg residues resulted in a sharp decrease in the mobility of methylene groups in side segments and in the main chain of ε-Lys inner segments. We suggest that this unexpected effect is caused by a guanidine–guanidine pairing effect in water, which leads to entanglements between dendrimer branches.


Last updated on 2020-27-03 at 13:02