A1 Journal article (refereed), original research

Characterization of Cellulase-Treated Fibers and Resulting Cellulose Nanocrystals Generated through Acid Hydrolysis

Open Access publication

Publication Details
Authors: Beyene Dawit, Chae Michael, Dai Jing, Danumah Christophe, Tosto Frank, Demesa Abayneh, Bressler David C
Publisher: MDPI
Publication year: 2018
Language: English
Related Journal or Series Information: Materials
Volume number: 11
Issue number: 8
ISSN: 1996-1944
JUFO-Level of this publication: 1
Permanent website address: https://www.mdpi.com/1996-1944/11/8/1272
Open Access: Open Access publication


Integrating enzymatic treatment and acid hydrolysis potentially improves the economics of cellulose nanocrystal (CNC) production and demonstrates a sustainable cellulosic ethanol co-generation strategy. In this study, the effect of enzymatic treatment on filter paper and wood pulp fibers, and CNCs generated via subsequent acid hydrolysis were assessed. Characterization was performed using a pulp quality monitoring system, scanning and transmission electron microscopies, dynamic light scattering, X-ray diffraction, and thermogravimetric analysis. Enzymatic treatment partially reduced fiber length, but caused swelling, indicating simultaneous fragmentation and layer erosion. Preferential hydrolysis of less ordered cellulose by cellulases slightly improved the crystallinity index of filter paper fiber from 86% to 88%, though no change was observed for wood pulp fibre. All CNC colloids were stable with zeta potential values below −39 mV and hydrodynamic diameters ranging from 205 to 294 nm. Furthermore, the temperature for the peak rate of CNC thermal degradation was generally not affected by enzymatic treatment. These findings demonstrate that CNCs of comparable quality can be produced from an enzymatically-mediated acid hydrolysis biorefining strategy that co-generates fermentable sugars for biofuel production.

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Last updated on 2019-13-03 at 12:00