A1 Journal article (refereed), original research

Numerical simulation of biomass growth in OKTOP®9000 reactor at industrial scale

Open Access hybrid publication

Publication Details
Authors: Gradov Dmitry, Han Mei, Tervasmäki Petri, Latva-Kokko Marko, Vaittinen Johanna, Pihlajamäki Arto, Koiranen Tuomas
Publisher: ACS American Chemical Society
Publication year: 2018
Language: English
Related Journal or Series Information: Industrial && Engineering Chemistry Research
Volume number: 57
Issue number: 40
Start page: 13300
End page: 13311
Number of pages: 12
ISSN: 0888-5885
eISSN: 1520-5045
JUFO-Level of this publication: 1
Open Access: Open Access hybrid publication


Computational fluid dynamics is a powerful method for scale-up of reactors although it is still challenging to fully embrace hydrodynamics and biological complexities. In this article, an aerobic fermentation of Pichia pastoris cells is modeled in a batch OKTOP®9000 reactor. The 800 m3 industrial scale reactor is equipped with a radial impeller, designed by Outotec Oy for gas dispersion in the draft tube reactor. Measured Np of the impeller is used in hydrodynamics validation. The resolved energy dissipation rate is compensated, and its influence on mass transfer is analyzed and discussed. Gas−liquid drag force is modified to simulate effects of liquid turbulence and bubble swarms. Resolved steady state multiphase hydrodynamics is used to simulate the fermentation process. Temporal evolution of species concentrations is compared to experimental data measured in a small copy of the reactor at lab scale (14 L). The effect of oxygenation on the P. pastoris cells cultivation is considered.

Last updated on 2019-13-03 at 12:00